Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FLI1

Cell type

Cell type Class
Bone
Cell type
A-673
Primary Tissue
Skeletal Muscle
Tissue Diagnosis
Rhabdomyosarcoma

Attributes by original data submitter

Sample

source_name
FLI1 ChIP in A673 transduced with KRAB-ZFA7
antibody
FLI1 antibody (Abcam, cat# ab15289, lot# GR3229309-6)
cell line
A673
treatment
transduced with KRAB-ZFA7

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, chromatin from 1% formaldehyde-fixed cells were fragmented to 200-500 bps by sonication for 5-6 mins using the Branson sonifer SFX250 ChIP-seq: Sequencing libraries were prepared with 1-5 ng of ChIP DNA samples and input sample using the Ovation Ultralow System V2 kit (Nugen). Libraries were sequenced with single-end (SE) 50-75 cycles on an Illumina Nextseq 500 Illumina genome analyzer

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
23241018
Reads aligned (%)
97.4
Duplicates removed (%)
18.5
Number of peaks
55952 (qval < 1E-05)

hg19

Number of total reads
23241018
Reads aligned (%)
96.8
Duplicates removed (%)
19.2
Number of peaks
55904 (qval < 1E-05)

Base call quality data from DBCLS SRA